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KMID : 0880220090470050657
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Journal of Microbiology
2009 Volume.47 No. 5 p.657 ~ p.662
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NOTE] Evidence Against the Physiological Role of Acetyl Phosphate in the Phosphorylation of the ArcA Response Regulator in Escherichia coli
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Liu Xueqiao
Gabriela R. Pena Sandoval
Dimitris Georgellis
Barry L. Wanner
Won Seok-Jung
Kwon Oh-Suk
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Abstract
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The Arc two-component signal transduction system of Escherichia coli comprises the ArcB sensor kinase and the ArcA response regulator. Under anoxic growth conditions, ArcB autophosphorylates and transphosphorylates ArcA, which, in turn, represses or activates its target operons. ArcA has been shown to be able to autophosphorylate in vitro at the expense of acetyl-P. Here, the in vivo effect of acetyl phosphate on the redox signal transduction by the Arc system was assessed. Our results indicate that acetyl phosphate can modulate the expression of ArcA-P target genes only in the absence of ArcB. Therefore, the acetyl phosphate dependent ArcA phosphorylation route does not seem to play a significant role under physiological conditions.
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KEYWORD
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acetyl phosphate, Arc two-component signal transduction, ArcA response regulator, E. coli
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